data acquisition analysis platform Search Results


scanarray microarray analysis system  (Revvity)
90
Revvity scanarray microarray analysis system
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Scanarray Microarray Analysis System, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/PerkinElmer+Signals+Screening-Assay+Data+Management+and+Analysis+Platform/pmc04410939-68-16-20
Average 90 stars, based on 1 article reviews
scanarray microarray analysis system - by Bioz Stars, 2026-10
90/100 stars
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sutterpatch data acquisition software  (Sutter Instrument Company)
93
Sutter Instrument Company sutterpatch data acquisition software
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Sutterpatch Data Acquisition Software, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/Dendrite+Data+Acquisition+and+Analysis+System/pmc12416546-75-13-17
Average 93 stars, based on 1 article reviews
sutterpatch data acquisition software - by Bioz Stars, 2026-10
93/100 stars
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electrophysiology device neuroexam m-800  (MedCom GmbH)
90
MedCom GmbH electrophysiology device neuroexam m-800
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Electrophysiology Device Neuroexam M 800, supplied by MedCom GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/neuroexam+m+800+data+acquisition+analysis+system/pm30071379-127-43-47
Average 90 stars, based on 1 article reviews
electrophysiology device neuroexam m-800 - by Bioz Stars, 2026-10
90/100 stars
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po-ne-mah digital data-acquisition and -analysis system  (Gould Instrument Systems Inc)
90
Gould Instrument Systems Inc po-ne-mah digital data-acquisition and -analysis system
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Po Ne Mah Digital Data Acquisition And Analysis System, supplied by Gould Instrument Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/po+ne+mah+digital+data+acquisition+and++analysis+system/pm10066710-64-24-27
Average 90 stars, based on 1 article reviews
po-ne-mah digital data-acquisition and -analysis system - by Bioz Stars, 2026-10
90/100 stars
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microscope control, data acquisition, and image analysis  (universal imaging inc)
90
universal imaging inc microscope control, data acquisition, and image analysis
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Microscope Control, Data Acquisition, And Image Analysis, supplied by universal imaging inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/microscope+control++data+acquisition++and+image+analysis/pmc01420652-243-2-11
Average 90 stars, based on 1 article reviews
microscope control, data acquisition, and image analysis - by Bioz Stars, 2026-10
90/100 stars
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acqknowledge waveform analysis function  (BIOPAC)
90
BIOPAC acqknowledge waveform analysis function
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Acqknowledge Waveform Analysis Function, supplied by BIOPAC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/waveform+data+acquisition+analysis+software+acqknowledge/pmc02290527-97-15-19
Average 90 stars, based on 1 article reviews
acqknowledge waveform analysis function - by Bioz Stars, 2026-10
90/100 stars
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tail-cuff plethysmography data acquisition analysis software  (BIOPAC)
90
BIOPAC tail-cuff plethysmography data acquisition analysis software
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Tail Cuff Plethysmography Data Acquisition Analysis Software, supplied by BIOPAC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/tail+cuff+plethysmography+data+acquisition+analysis+software/pmc07675192-148-9-16
Average 90 stars, based on 1 article reviews
tail-cuff plethysmography data acquisition analysis software - by Bioz Stars, 2026-10
90/100 stars
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data acquisition system po-ne-mah; version 3  (Gould Instrument Systems Inc)
90
Gould Instrument Systems Inc data acquisition system po-ne-mah; version 3
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Data Acquisition System Po Ne Mah; Version 3, supplied by Gould Instrument Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/po+ne+mah+data+acquisition+software+system+ponemah+physiology+platform/10__1111_slash_j__1469___7793__2004__tb00489__x-100-34-40
Average 90 stars, based on 1 article reviews
data acquisition system po-ne-mah; version 3 - by Bioz Stars, 2026-10
90/100 stars
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computer-driven real-time data acquisition/ analysis system  (Dasytec USA Inc)
90
Dasytec USA Inc computer-driven real-time data acquisition/ analysis system
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Computer Driven Real Time Data Acquisition/ Analysis System, supplied by Dasytec USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/computer+driven+real+time+data+acquisition+analysis+system/pm22634620-73-36-43
Average 90 stars, based on 1 article reviews
computer-driven real-time data acquisition/ analysis system - by Bioz Stars, 2026-10
90/100 stars
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tempo-seqr data analysis platform  (BioSpyder Technologies)
90
BioSpyder Technologies tempo-seqr data analysis platform
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Tempo Seqr Data Analysis Platform, supplied by BioSpyder Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/tempo+seqr+data+analysis+platform/pm30794557-117-7-11
Average 90 stars, based on 1 article reviews
tempo-seqr data analysis platform - by Bioz Stars, 2026-10
90/100 stars
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pro microplate data acquisition and analysis software  (SoftMax Inc)
90
SoftMax Inc pro microplate data acquisition and analysis software
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Pro Microplate Data Acquisition And Analysis Software, supplied by SoftMax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/pro+microplate+data+acquisition+and+analysis+software/pmc09794825-118-24-18
Average 90 stars, based on 1 article reviews
pro microplate data acquisition and analysis software - by Bioz Stars, 2026-10
90/100 stars
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grass polyviewtm software version 2.1  (AstroNova)
90
AstroNova grass polyviewtm software version 2.1
A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different <t>microarray</t> slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).
Grass Polyviewtm Software Version 2.1, supplied by AstroNova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+acquisition+analysis+platform/grass+polyview+data+acquisition+and+analysis+system/pm15653707-45-0-5
Average 90 stars, based on 1 article reviews
grass polyviewtm software version 2.1 - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different microarray slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).

Journal: PLoS ONE

Article Title: MicroRNA-27a Contributes to Rhabdomyosarcoma Cell Proliferation by Suppressing RARA and RXRA

doi: 10.1371/journal.pone.0125171

Figure Lengend Snippet: A ) Experimental design: the miRNA population from each cell line was compared to a common reference sample consisting of a balanced mixture of eight small RNA samples (< 200 nt) prepared from the same cell lines. Two replicates of each experiment were performed using different microarray slides in which sample and reference RNAs, labeled with Cy3 (gray arrow) or Cy5 (black arrow) fluorochromes, were crossed in both combinations (dye-swapping procedure). B ) Heat map of 16 discriminant miRNAs between PAX3/FOXO1 positive ARMS (in gray on the right, RH4, RH30, RH28) and negative (in dark grey on the left, RD, SMS-CTR, RH18, RH36, CCA) RMS cell lines identified by SAM analysis ( rows : miRNAs; columns : RMS cell lines). A color-coded scale for the normalized expression values was used as follows: yellow and blue represent high and low expression levels in RMS cell lines with respect to a reference sample (a pool of eight RMS cell lines). The expression level of each miRNA was calculated as the ln (RMS cell line/Pool). C ) Expression levels of miR-199a, miR-23a and miR-27a in eight RMS cell lines obtained with qRT-PCR. Two independent experiments were performed in triplicate. Results are shown as relative expression ratio obtained with the 2 -ΔΔCt method. RNU6B was used as reference miRNA. Vertical bars represent the 95% confidence interval (IC).

Article Snippet: Array scanning was carried out using a GSI Lumonics LITE dual confocal laser scanner with a ScanArray Microarray Analysis System (Perkin Elmer, Waltham, MA), and raw images were analyzed with QuantArray Analysis Software (GSI Lumonics, Ottawa, Canada).

Techniques: Microarray, Labeling, Expressing, Quantitative RT-PCR